What is done after staining in Modified Knott's technique?

What is done after staining in Modified Knott's technique?

• A. Transfer to glass slide
• B. Apply coverslip
• C. Stain again with hematoxylin
• D. Freeze it

Answer: (A)

In the Modified Knott’s technique, after staining the concentrated sediment, you move the material onto a glass slide to create a smear for microscopic examination. Transferring to the slide concentrates the organisms in a small, viewable field and allows the stained microfilariae to be seen clearly under the microscope. Freezing would disrupt or destroy the sample, re-staining with hematoxylin isn’t part of this protocol, and while some setups may use a coverslip, the essential next step is placing the stained material on a slide for observation.

In the Modified Knott’s technique, after staining the concentrated sediment, you move the material onto a glass slide to create a smear for microscopic examination. Transferring to the slide concentrates the organisms in a small, viewable field and allows the stained microfilariae to be seen clearly under the microscope. Freezing would disrupt or destroy the sample, re-staining with hematoxylin isn’t part of this protocol, and while some setups may use a coverslip, the essential next step is placing the stained material on a slide for observation.